Scientist- Cell-Based Assays (384 Well Format) Job at AllSTEM Connections, Rahway, NJ

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  • AllSTEM Connections
  • Rahway, NJ

Job Description

Job Title: Scientist, Cell-Based Assays (384 Well Format)

Duration: 12+ Months

Location: Rahway NJ 07065

Requirements:

  • BS/MS/PHD in Biology, Pharmacology, Biochemistry, or related field; BS with 5+ years, Masters with 3+ years and PHD with 1+ year of industry or equivalent lab experience.
  • Proficiency in mammalian cell culture and 384-well assay workflows
  • Experience with plate readers and assay platforms (e.g., cAMP, HTRF, luminescent assays)
  • Strong data analysis skills: 4PL curve fitting, EC50/IC50 determination, and curve QC (e.g., R^2, top/bottom constraints)
  • Detail-oriented, organized, and comfortable in a fast-paced, high-throughput environment

Nice to Have:

  • Experience with automation/LIQ handlers, LIMS/ELN, and statistical QC (Z’, CV, S/B)
  • Familiarity with GraphPad Prism, ActivityBase, or equivalent analysis tools
  • Hands-on experience with Echo acoustic dispensing.

Note:

  • Onsite assignment @ Rahway,NJ.
  • 100% Lab based role.

Overview: We’re seeking a hands-on scientist to execute high-throughput cell-based assays in 384-well format. You will culture and plate cells, dispense compounds using the Echo, run diverse detection readouts, and analyze dose–response data to deliver high-quality EC50 results for discovery programs.

Key Responsibilities:

  • Maintain, expand, and plate mammalian cell lines for 384-well assays
  • Set up and execute assays with compound dispensing via Labcyte/Beckman Echo
  • Run and optimize detection formats (e.g., luminescence, cAMP, HTRF/TR-FRET, fluorescence)
  • Generate dose–response curves (4-parameter logistic fits) and report EC50/IC50 with QC
  • Troubleshoot assay performance, optimize Z’ factors, and document SOPs/results
  • Collaborate with discovery teams to prioritize studies and timelines

Additional responsibilities may include setting up western blot assays and learning how to run in-cell westerns

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